Journal: ACS Applied Materials & Interfaces

Article Title: Zinc-Doped Antibacterial Coating as a Single Approach to Unlock Multifunctional and Highly Resistant Titanium Implant Surfaces

doi: 10.1021/acsami.4c21875

Figure Lengend Snippet: Schematic diagram of the experimental design. SEM = Scanning electron microscopy; EDS = Energy-dispersive X-ray spectrometry; XRD = X-ray diffractometry; CLSM = Confocal laser scanning microscopy; XPS = X-ray photoelectron spectroscopy; FTIR = Fourier transform infrared spectroscopy; WCA = Water contact angle; OCP = Open circuit potential; EIS = Electrochemical impedance spectroscopy; CFU = Colony forming units; ICP = Inductively coupled plasma. Created with BioRender.com (License number: AV27FQ9RWZ).

Article Snippet: For the surface area and topography analysis, a noncontact three-dimensional Confocal Laser Scanning Microscope (CLSM) (VK-X200 series, Keyence, Osaka, Japan) was utilized to acquire images, which were then processed using the VK-Analyzer software (Keyence v3.3.0.0, Osaka, Japan) ( n = 3/group).

Techniques: Electron Microscopy, Confocal Laser Scanning Microscopy, Spectroscopy, Fourier Transform Infrared Spectroscopy, Impedance Spectroscopy, Clinical Proteomics

Journal: ACS Applied Materials & Interfaces

Article Title: Zinc-Doped Antibacterial Coating as a Single Approach to Unlock Multifunctional and Highly Resistant Titanium Implant Surfaces

doi: 10.1021/acsami.4c21875

Figure Lengend Snippet: Topographic characterization of control and PEO-treated surfaces. (a) Scanning electron microscopy (SEM) images showing top view surface morphology (scale bar = 10 μm, magnification 1000 × , 15 kV). (b) Energy-dispersive X-ray spectroscopy (EDS) surface maps, highlighting the atomic percentage of individual elements (magnification 2000 × , 10 kV). (c) Cross-sectional SEM analysis of the PEO coatings (scale bar = 10 μm, magnification 2000 × , 10 kV). (d) Cross-sectional EDS maps indicating the spatial distribution of elements across the coating layers (scale bar = 10 μm, magnification 2000 × , 10 kV). (e) Three-dimensional representations of the surface topography obtained via confocal laser scanning microscopy (magnification 50 × ), including the average surface area measurements.

Article Snippet: For the surface area and topography analysis, a noncontact three-dimensional Confocal Laser Scanning Microscope (CLSM) (VK-X200 series, Keyence, Osaka, Japan) was utilized to acquire images, which were then processed using the VK-Analyzer software (Keyence v3.3.0.0, Osaka, Japan) ( n = 3/group).

Techniques: Control, Electron Microscopy, Spectroscopy, Confocal Laser Scanning Microscopy

Journal: ACS Applied Materials & Interfaces

Article Title: Zinc-Doped Antibacterial Coating as a Single Approach to Unlock Multifunctional and Highly Resistant Titanium Implant Surfaces

doi: 10.1021/acsami.4c21875

Figure Lengend Snippet: Structural and physicochemical properties characterization of cpTi and PEO-treated samples. (a) Pore area and (b) pore density was determined from SEM micrographs at 2000 × magnification ( n = 3). (c) Coating thickness of cpTi and PEO samples ( n = 1, measurements taken at 5 distinct points). (d) Surface area calculated from three-dimensional CLSM images ( n = 3). (e) Cumulative zinc ions released in PBS over 7 days ( n = 3). Surface roughness measurements ( n = 5) for cpTi and PEO groups are represented as (f) Ra (average roughness), (g) Rt (maximum height of the profile), (h) Rq (root-mean-square roughness), and (i) Rz (average maximum height). (j) Water contact angle values ( n = 5) and representative images of water droplets on control and experimental surfaces. Data are expressed as mean ± standard deviation. Statistically significant differences between groups are indicated by symbols, with * representing p < 0.05 and ** representing p < 0.001, as determined by Tukey HSD test.

Article Snippet: For the surface area and topography analysis, a noncontact three-dimensional Confocal Laser Scanning Microscope (CLSM) (VK-X200 series, Keyence, Osaka, Japan) was utilized to acquire images, which were then processed using the VK-Analyzer software (Keyence v3.3.0.0, Osaka, Japan) ( n = 3/group).

Techniques: Control, Standard Deviation